count source_label source_id relationship target_label target_id entity_type solr_id publication_id sentences 4 UNIPROT:Q9BZD4 activates spindle localization GO:0051653 Protein 5462cc18-c46d-11e5-85e4-001a4ae51246 PMC4207087 Consistent with our previously published data, small interfering RNA (siRNA) depletion of the outer kinetochore component Nuf2 abrogates Kif15 spindle localization (Figure 4F) by preventing K-fiber formation [1, 27]. 4 FPLX:Kinetochore activates spindle localization GO:0051653 ProteinFamily 5462cc18-c46d-11e5-85e4-001a4ae51246 PMC4207087 Consistent with our previously published data, small interfering RNA (siRNA) depletion of the outer kinetochore component Nuf2 abrogates Kif15 spindle localization (Figure 4F) by preventing K-fiber formation [1, 27]. 4 UNIPROT:Q96BD8 activates spindle localization GO:0051653 Protein 1e004f50-3910-11e8-8636-001a4a160175 PMC5726585 In addition, although the Ska1 MTBD is necessary and sufficient for Ska1 complex microtubule binding and spindle localization [2], we note that recent work has also suggested a contribution to microtubule interactions from Ska3 [28]. 4 UNIPROT:Q9XF43 activates spindle localization GO:0051653 Protein 57aba0ba-cc1b-11e5-b0dd-001a4ae51247 9635190 We wanted to know how the carboxy-terminal region of Cut1 contributed to spindle localization. 2 UNIPROT:Q9ULW0 activates spindle localization GO:0051653 Protein 20a8b476-c46f-11e5-9da3-001a4ae51247 25291641 Furthermore, TPX2 knockdown eliminates KIF15 spindle localization[3]. 2 UNIPROT:Q9ULW0 activates spindle localization GO:0051653 Protein 5462cc18-c46d-11e5-85e4-001a4ae51246 PMC4207087 In support of this notion, TPX2 depletion abrogates Kif15 spindle localization [3, 4]. 2 UNIPROT:P38283 activates spindle localization GO:0051653 Protein 3d4284e0-edaa-11e5-9b35-001a4ae51246 PMC4749444 For example, Cdc14-dependent dephosphorylation of Sli15, a subunit of the chromosomal passenger complex, promotes Ipl1 spindle localization (Mirchenko and Uhlmann, 2010; Pereira and Schiebel, 2003). 2 UNIPROT:O00139 inhibits spindle localization GO:0051653 Protein 8b9095c4-351a-11e8-8636-001a4a160175 28633016 We demonstrate that MLL and WDR5 are required for spindle localization of Kif2A, while mutation of the Win motif of Kif2A abolishes its spindle localization. 2 UNIPROT:P49913 activates spindle localization GO:0051653 Protein e3253fa2-c47d-11e5-91a7-001a4ae51247 PMC3020106 Spindle localization of MAD2L2 was abrogated by CAMP depletion (Supplementary Figure S2A), although the role of MAD2L2 on the spindle has not been clarified yet (Medendorpet al, 2009).|||We found that spindle localization of MAD2L2 was abolished by depletion of CAMP (Supplementary Figure S2A), while the expression of MAD2L2 was not altered (data not shown), suggesting that CAMP is required for the spindle localization of MAD2L2. 1 spindle localization GO:0051653 activates UNIPROT:P55265 Protein e0584044-d47c-11e5-90d3-001a4ae51247 PMC4338354 "An ADAR-containing complex is necessary for spindle localization of dsRNA To identify factors that mediate localization of exogenous dsRNA to meiotic spindles, we performed a biochemical screen for proteins in the egg extract that bind dsRNA but not ssRNA." 1 spindle localization GO:0051653 inhibits GO:0051323 Phenotype 2b9a61ca-bc47-11e5-9b9d-001a4ae51247 PMC3135399 Because localization of the CPC to MTs stabilizes the anaphase spindle (Pereira and Schiebel, 2003), we hypothesized that premature spindle localization of the CPC may perturb MT dynamics necessary for proper metaphase chromosome positioning. 1 spindle localization GO:0051653 activates GO:0051179 Phenotype 0f28ea06-9a1b-11ee-bde4-0050569a1f61 10.1007/s00018-021-03891-x The D254A mutation also consistently improved spindle localization of Cin8ΔL8, inducing SPB localization in monopolar spindles (similar to WT Cin8) and spindle attachment prior to and during anaphase B (Fig.1D, G). 1 spindle localization GO:0051653 inhibits GO:0051303 Phenotype 2b9a61ca-bc47-11e5-9b9d-001a4ae51247 PMC3135399 Because localization of the CPC to MTs stabilizes the anaphase spindle (Pereira and Schiebel, 2003), we hypothesized that premature spindle localization of the CPC may perturb MT dynamics necessary for proper metaphase chromosome positioning. 1 GO:0006810 activates spindle localization GO:0051653 Phenotype 29bf7aaa-c473-11e5-9da3-001a4ae51247 PMC2909321 We speculate that spindle localization of FAM29A and NEDD1 in wild-type cells may be mediated by an active transport of the FAM29A-NEDD1 complex toward the plus ends of spindle MTs and this transport is absent upon inhibition of Plk1. 1 PF:PF16796 inhibits spindle localization GO:0051653 ProteinFamily f21e277e-bc41-11e5-9b9d-001a4ae51247 PMC3967068 Spindle localization of Sli15 before anaphase is prevented by both Cdk1- and Ipl1-dependent phosphorylation of the microtubule-binding domain in Sli15 (Nakajima et al.2011; Pereira and Schiebel2003). 1 UNIPROT:Q15746 activates spindle localization GO:0051653 Protein 55d2b31e-37fa-11e6-aaca-001a4ae51246 25904014 "Inhibition of MEK/12 with U0126 or of MLCK with ML-7 Causes Loss of Spindle Localization Adjacent to the Cortex in a Subset of Drug-Treated Metaphase II Eggs Eggs were treated with the solvent control DMSO, 50 μM U0126, or 15 μM ML-7, and then imaged by time-lapse microscopy or fixed after 3 h treatment and stained to label the DNA and meiotic spindle as well as mitotic phospho-proteins (addressed in more detail below)." 1 UNIPROT:P53350 activates spindle localization GO:0051653 Protein ea625826-c46a-11e5-a92e-001a4ae51246 25128564 Furthermore, Plk1 promotes the centrosome and spindle localization of ASAP, and Plk1-mediated phosphorylation of ASAP is sufficient to maintain spindle pole integrity in a microtubule-dependent manner; however, the underlying mechanism remains unclear (Eot-Houllier et al., 2010). 1 UNIPROT:Q8NHV4 inhibits spindle localization GO:0051653 Protein 20e0c3aa-bc47-11e5-8abe-001a4ae51246 PMC2483532 Furthermore, mutation of a phosphorylation site in NEDD1 abolishes its spindle localization as well as spindle assembly. 1 UNIPROT:Q7Z4H7 activates spindle localization GO:0051653 Protein 29bf7aaa-c473-11e5-9da3-001a4ae51247 PMC2909321 We speculate that spindle localization of FAM29A and NEDD1 in wild-type cells may be mediated by an active transport of the FAM29A-NEDD1 complex toward the plus ends of spindle MTs and this transport is absent upon inhibition of Plk1. 1 GO:0051323 activates spindle localization GO:0051653 Phenotype 55d2b31e-37fa-11e6-aaca-001a4ae51246 25904014 "Treatment of metaphase II eggs with the ARP2/3 complex inhibitor CK-666 induces a rapid loss of cortical spindle localization, evident within a few minutes of exposing eggs to CK-666 [74], whereas this rapid loss of spindle localization was not detected in eggs treated with blebbistatin, an inhibitor of the myosin-II ATPase [74]." 1 UNIPROT:Q08050 inhibits spindle localization GO:0051653 Protein 8b9095c4-351a-11e8-8636-001a4a160175 28633016 We demonstrate that MLL and WDR5 are required for spindle localization of Kif2A, while mutation of the Win motif of Kif2A abolishes its spindle localization. 1 UNIPROT:Q5SW79 activates spindle localization GO:0051653 Protein d2dd8b32-bc3e-11e5-8d2d-001a4ae51247 PMC3521686 Future biochemical analyses will be required to determine whether Cep170 modulates Kif2b activity in addition to promoting its spindle localization. 1 UNIPROT:P63000 activates spindle localization GO:0051653 Protein 55d2b31e-37fa-11e6-aaca-001a4ae51246 25904014 "Expression of a dominant-negative version of the GTPase Rac1 in metaphase II eggs induces either a loss of cortical anchoring of one spindle pole or a gradual loss of cortical spindle localization [73]." 1 FPLX:Dynein inhibits spindle localization GO:0051653 ProteinFamily 07cb5e14-351e-11e8-9192-001a4a160175 PMC5584168 We wondered whether the observed premature spindle localization of Ipl1-3GFP inshe1Δwas caused by the disruption of dynein pathways. 1 UNIPROT:Q15398 inhibits spindle localization GO:0051653 Protein 2f8f83c6-dc7e-11ea-8b11-001a4a160176 PMC6177585 "Immunofluorescence and line profile analyses showed that depletion of Nuf2 did not abolish the attachment of TACC3 to microtubules, whereas HURP depletion could modulate the spindle localization of TACC3 (Fig. S5,BandC)." 1 FPLX:Kinesin activates spindle localization GO:0051653 ProteinFamily 1aaa11d8-c9ff-11e5-b88f-001a4ae51247 15367580 Localization of BimC kinesins to spindles in vertebrate cells has been linked to phosphorylation of a Cdk1 target site in the conserved BimC Box near the carboxyl tail region of these kinesins (Blangy et al., 1995;Sawin and Mitchison, 1995;Sharp et al., 1999a), postulated to elicit or strengthen intrinsic microtubule binding activity and spindle localization. 1 UNIPROT:P06493 inhibits spindle localization GO:0051653 Protein f21e277e-bc41-11e5-9b9d-001a4ae51247 PMC3967068 Spindle localization of Sli15 before anaphase is prevented by both Cdk1- and Ipl1-dependent phosphorylation of the microtubule-binding domain in Sli15 (Nakajima et al.2011; Pereira and Schiebel2003). 1 UNIPROT:Q9Y6A5 activates spindle localization GO:0051653 Protein 281c9e80-c478-11e5-91a7-001a4ae51247 PMC3049211 Examples of results that are incompatible with this model are (i) overexpression of GFP–CHC did not cause more clathrin to accumulate at the spindle and it did not influence the spindle localisation of TACC3 or ch‐TOG, (ii) overexpression of TACC3 increased the spindle localisation of ch‐TOG and clathrin and (iii) blocking spindle recruitment of TACC3 by inhibition of aurora‐A kinase or by mutating TACC3 inhibited the recruitment of clathrin to the spindle. 1 UNIPROT:P53667 activates spindle localization GO:0051653 Protein 05e28074-c481-11e5-85e4-001a4ae51246 22328514 Active LIMK2 (T484E), but not active LIMK1 (T508E), restored TPPP spindle-localization upon treatment with Y27632 and C3 transferase (Fig. 5C). 1 UNIPROT:Q02241 activates spindle localization GO:0051653 Protein 40c0ae38-c471-11e5-9cc6-001a4ae51246 19339549 Depletion of the centralspindlin component MKLP1 prevents central spindle localization of Ect2; however, RhoA, F-actin and myosin have been shown to accumulate on the equatorial cell cortex indicating that their localization is regulated independently (Yuce et al., 2005). 1 FPLX:CK2 inhibits spindle localization GO:0051653 ProteinFamily 71cfbb7c-c47b-11e5-9cc6-001a4ae51246 21693590 Additionally, only a subset of CK2α is phosphorylated during normal mitotic progression (St-Denis et al., 2009), further limiting the proportion of kinase available for spindle localization. 1 UNIPROT:P41271 activates spindle localization GO:0051653 Protein 43e14b7a-c473-11e5-85e4-001a4ae51246 PMC2777925 "Instead, Nbl1-GFP often localized diffusely in the nucleoplasm incut17-275andpic1-T269cells (57 of 72 cells, 79%; and 58 of 88 cells, 66%, respectively;Figure 3, D and E), indicating that Bir1 and Pic1 are necessary for its proper spindle localization." 1 UNIPROT:Q96GD4 inhibits spindle localization GO:0051653 Protein 07cb5e14-351e-11e8-9192-001a4a160175 PMC5584168 Our experiments showed that She1 spindle localization was reduced by Ipl1 phosphorylation (Fig. 6, B and C), whereas Ipl1 relocalized to the spindle only upon anaphase onset (Fig. S4, A and B), suggesting that Ipl1 and She1 localization on the spindle might be anticorrelated in anaphase. 1 UNIPROT:Q96GD4 inhibits spindle localization GO:0051653 Protein f21e277e-bc41-11e5-9b9d-001a4ae51247 PMC3967068 Spindle localization of Sli15 before anaphase is prevented by both Cdk1- and Ipl1-dependent phosphorylation of the microtubule-binding domain in Sli15 (Nakajima et al.2011; Pereira and Schiebel2003). 1 UNIPROT:Q9NS87 activates spindle localization GO:0051653 Protein 86aa2d7c-5c49-11e7-9fde-001a4ae51247 PMC5221630 The results presented here demonstrate that the C-terminal 37 amino acids of TPX2 regulate in vitro motility of Kif15 and contribute to the spindle localization of Kif15 and Eg5-independent force generation by Kif15 in vivo. 1 UNIPROT:Q8N0X4 activates spindle localization GO:0051653 Protein ea28120e-bc3d-11e5-ac4e-001a4ae51246 PMC2865516 In order to determine if Clb/Cdc28 kinase activity is necessary for the spindle localization of Kip1 and Cin8, we examined localization both in Δclb1,2,3,4,5,6and in separateGAL1-SIC1Δ3Pstrains. 1 UNIPROT:Q9NQS7 activates spindle localization GO:0051653 Protein cb42ebea-bc40-11e5-9b9d-001a4ae51247 PMC3849490 In such a scenario, the INCENP mutants could cause premature spindle localization of the CPC, leading to activation of Aurora B on the spindle instead of at the centromere [40].