count source_label source_id relationship target_label target_id entity_type solr_id publication_id sentences 20 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily 06fb88cc-cb28-11e5-b419-001a4ae51246 11502750 To determine whether SR protein dephosphorylation is mediated by ceramide-activated protein phosphatasesin vivo, specifically PP1, we pretreated cells with the serine/threonine phosphatase inhibitors, okadaic acid (in vitroIC50: PP2A IC50= 0.1 nm, PP1 IC50= 10 nm), and calyculin A (in vitroIC50: PP2A IC50= 1 nm, PP1 IC50= 2 nm) followed by a 6-h treatment with 20 μmd-(e)-C6-ceramide.|||Calyculin A, but Not Okadaic Acid, Inhibits Ceramide-induced Dephosphorylation of SR Proteins To determine whether SR protein dephosphorylation is mediated by ceramide-activated protein phosphatasesin vivo, specifically PP1, we pretreated cells with the serine/threonine phosphatase inhibitors, okadaic acid (in vitroIC50: PP2A IC50= 0.1 nm, PP1 IC50= 10 nm), and calyculin A (in vitroIC50: PP2A IC50= 1 nm, PP1 IC50= 2 nm) followed by a 6-h treatment with 20 μmd-(e)-C6-ceramide.|||Furthermore, calyculin A, an inhibitor of both PP1 and PP2A, completely blocked ceramide- and FAS-induced SR protein dephosphorylation, while okadaic acid at concentrations that only affect PP2A in cells had no effect. 16 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily daa0f0e4-cb29-11e5-9aa0-001a4ae51247 11821394 These data suggest that the majority, but not all, of the temperature-dependent activity loss is due to protein dephosphorylation mediated by a microcystin-sensitive phosphatase. 16 MESH:D052998 activates protein dephosphorylation GO:0006470 Phenotype daa0f0e4-cb29-11e5-9aa0-001a4ae51247 11821394 These data suggest that the majority, but not all, of the temperature-dependent activity loss is due to protein dephosphorylation mediated by a microcystin-sensitive phosphatase. 16 CHEBI:38221 inhibits protein dephosphorylation GO:0006470 Chemical 5124c79a-cb2a-11e5-a6cd-001a4ae51247 12200446 Furthermore, SR protein dephosphorylation was also inhibited by fumonisin B1 pretreatment (Fig.6B) at the 2-h time point. 16 MESH:D002518 activates protein dephosphorylation GO:0006470 Phenotype 06fb88cc-cb28-11e5-b419-001a4ae51246 11502750 To determine whether SR protein dephosphorylation is mediated by ceramide-activated protein phosphatasesin vivo, specifically PP1, we pretreated cells with the serine/threonine phosphatase inhibitors, okadaic acid (in vitroIC50: PP2A IC50= 0.1 nm, PP1 IC50= 10 nm), and calyculin A (in vitroIC50: PP2A IC50= 1 nm, PP1 IC50= 2 nm) followed by a 6-h treatment with 20 μmd-(e)-C6-ceramide.|||Calyculin A, but Not Okadaic Acid, Inhibits Ceramide-induced Dephosphorylation of SR Proteins To determine whether SR protein dephosphorylation is mediated by ceramide-activated protein phosphatasesin vivo, specifically PP1, we pretreated cells with the serine/threonine phosphatase inhibitors, okadaic acid (in vitroIC50: PP2A IC50= 0.1 nm, PP1 IC50= 10 nm), and calyculin A (in vitroIC50: PP2A IC50= 1 nm, PP1 IC50= 2 nm) followed by a 6-h treatment with 20 μmd-(e)-C6-ceramide. 12 CHEBI:2365 inhibits protein dephosphorylation GO:0006470 Chemical 22da996c-cc17-11e5-b0dd-001a4ae51247 9654130 The finding that ABA mimics the previously reported OA-induced maintenance of S-type currents with zero ATP in the cytosol (Fig. 2) supports a hypothesis in which ABA down-regulates protein dephosphorylation events during stomatal closing inVicia fabaguard cells.|||Thus the presented data provide cell biological evidence that ABA can post-translationally inactivate protein dephosphorylation events, while at the same time ABA activates protein kinases[20–23]during signaling.|||In conclusion, the present study provides direct evidence for ABA regulation of S-type anion channels inVicia fabaguard cells in the absence of cytosolic ATP and implicates ABA down-regulation of protein dephosphorylation events during ABA signaling, in addition to previously reported ABA activation of protein phosphorylation events[13, 20–23]. 4 protein dephosphorylation GO:0006470 inhibits GO:0006303 Phenotype 7461485e-bbea-11e5-8abe-001a4ae51246 10.1016/j.dnarep.2006.12.007 These findings suggest that protein dephosphorylation diminishes dissociation of the NHEJ complex. 4 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 06fb88cc-cb28-11e5-b419-001a4ae51246 11502750 Furthermore, calyculin A, an inhibitor of both PP1 and PP2A, completely blocked ceramide- and FAS-induced SR protein dephosphorylation, while okadaic acid at concentrations that only affect PP2A in cells had no effect. 4 CHEBI:2365 inhibits protein dephosphorylation GO:0006470 Chemical 233db86a-0d7d-11f0-b759-0050569a791b 10.1016/S0981-9428(99)80044-8 These data, together with those showing that the phosphatase inhibitor okadaic acid also maintains the slow anion current in the absence of ATP[69], support a hypothesis in which ABA down-regulates protein dephosphorylation events during stomatal closure inV. 4 FPLX:Protease activates protein dephosphorylation GO:0006470 ProteinFamily 3a0298f8-3360-11e8-9fbf-001a4a160176 PMC9976551 More specifically, it appears that Man-A drives the collapse of H2O2tone which in turn, leads to un-repressed PPases and proteases, which then drive protein dephosphorylation and apoptosis. 4 CHEBI:41791 activates protein dephosphorylation GO:0006470 Chemical 06fb88cc-cb28-11e5-b419-001a4ae51246 11502750 Furthermore, calyculin A, an inhibitor of both PP1 and PP2A, completely blocked ceramide- and FAS-induced SR protein dephosphorylation, while okadaic acid at concentrations that only affect PP2A in cells had no effect. 4 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 82a17c5e-ca5e-11e5-a3f7-001a4ae51246 14576165 To investigate the role of PS1 phosphorylation on its turnover during apoptosis, the cells were incubated with okadaic acid (OA) that inhibits protein dephosphorylation by protein phosphatases 1 and 2A. 4 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 2b6552d6-bbee-11e5-9b9d-001a4ae51247 10.1016/j.brainres.2006.05.092 To test whether phosphorylation of rabphilin was maximal in DKO nerve terminals, we applied okadaic acid for 5 min at a concentration of 1 μM. Under these conditions, okadaic acid is an effective inhibitor of synaptic protein dephosphorylation (Lonart et al., 2003; Lonart and Südhof, 2000; Simsek-Duran et al., 2004; Foletti et al., 2001). 4 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily 539a6ad0-bc51-11e5-ac4e-001a4ae51246 10.1016/j.jneumeth.2005.09.015 Total protein was extracted by using the lysis buffer with inclusion of phosphatase inhibitors to maintain the protein phosphorylation status or with inclusion of recombinant protein phosphatase-1 (PP1) instead of phosphatase inhibitors to induce the protein dephosphorylation reaction, followed by processing for immunoprecipitation (IP) with rabbit polyclonal antibody reacting with all 14-3-3 isoforms (K-19) or with normal rabbit IgG. 3 UNIPROT:Q8NHJ6 activates protein dephosphorylation GO:0006470 Protein b9230488-c472-11e5-8491-001a4ae51247 PMC2787346 In contrast, treatment of cells with SP for 10 min significantly reversed LILRB4-mediated inhibition of TNFα production by 80% (Fig. 5C) and markedly reversed LILRB4-mediated protein dephosphorylation (Fig. 5D).|||LILRB4-mediated Protein Dephosphorylation and Inhibition of Cytokine Production via Induction of Phosphatases To assess whether LILRB4-mediated inhibition of protein-tyrosine kinase phosphorylation and the production of TNFα was through recruitment and activation of protein phosphatases, THP-1 cells were pretreated with 11 μmSS or 100 μmSP.|||However, treatment of cells with SS for 72 h significantly reversed the inhibitory effect of LILRB4 on TNFα production by up to 90% (Fig. 5A), despite its failure to reverse LILRB4-mediated protein dephosphorylation (Fig. 5B). 2 protein dephosphorylation GO:0006470 inhibits GO:0007049 Phenotype fb7fd6ca-bc26-11e5-8abe-001a4ae51246 10.1016/j.mehy.2006.10.043 Because protein phosphorylation is reversible, cell-cycle checkpoint can be reversed by protein dephosphorylation to allow the resumption of cell cycle after DNA repair. 2 CHEBI:29101 inhibits protein dephosphorylation GO:0006470 Chemical f89bff5c-c8e6-11e5-9ad8-001a4ae51247 17259169 Sodium orthovanadate (1 mm) was added to the lysis buffer to prevent protein dephosphorylation. 2 MESH:D000431 activates protein dephosphorylation GO:0006470 Phenotype bcdfbc24-ca5d-11e5-a3f7-001a4ae51246 12536146 Indeed, ethanol has been shown to accelerate the kinetics of protein dephosphorylation by protein-tyrosine phosphatases (25). 2 CHEBI:43945 inhibits protein dephosphorylation GO:0006470 Chemical 21753f86-0d7c-11f0-85c3-0050569a1f61 10.1016/S8756-3282(99)00070-8 Bisphosphonates also inhibited the hydrolysis of a c-srcpeptide substrate by PTPmeg1, although there was no correlation between the inhibitory potency and the antiresorptive potency of the bisphosphonates.37Because tiludronate has also been shown to increase the level of protein phosphorylation in osteoclasts by inhibiting protein phosphatase activity,33it is possible that inhibition of protein dephosphorylation by bisphosphonates, and hence interference with signal transduction in osteoclasts, may contribute to loss of osteoclast activity. 2 UNIPROT:P10145 inhibits protein dephosphorylation GO:0006470 Protein 3ad3ac3a-cc75-11e5-aade-001a4ae51247 PMC7164083 After different light treatments, samples were immediately frozen in liquid nitrogen and stored at −80°C. Thylakoid membranes ofSynechocystiswere isolated according to Tyystjärvi et al.[21]in the presence of 10 mM NaF to inhibit thylakoid protein dephosphorylation. 2 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 356f8946-c6b1-11ee-9133-0050569a1f61 10.1016/j.scitotenv.2023.167167 The inhibition of PP1 and PP2A activity induces protein dephosphorylation; however, after the protein is dephosphorylated, the activity of PP1 and PP2A cannot be restored. 2 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 69d183e8-7744-11e6-b946-001a4ae51247 10.1074/jbc.272.27.16729 To verify that PP2A-induced inhibition of telomerase activity involves protein dephosphorylation, nuclear telomerase extracts were coincubated with PP2A and various concentrations of okadaic acid, which binds to the catalytic motif of PP2A (32). 2 GO:0007049 activates protein dephosphorylation GO:0006470 Phenotype 90cb3eaa-bc49-11e5-9b9d-001a4ae51247 PMC2217494 Cell cycle– and growth-dependent reduction of GCK-3 activity below a critical level would lead to net protein dephosphorylation and activation of CLH-3b in maturing oocytes. 2 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily 77abdb34-3387-11e8-9192-001a4a160175 27633557 CaN-induced protein dephosphorylation in the hippocampus was exposed to impair the reinforcing effects of chronic d-amphetamine and morphine treatment, suggesting that hippocampus-dependent learning and memory may be involved in the development of drug addiction[4]. 2 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily 1ba6eb7a-ca03-11e5-9b70-001a4ae51247 15746091 These data strongly suggest that an increase in intracellular Ca2+by TRPV1 leads to down-regulation of HVACCs by protein dephosphorylation mediated by Ca2+-dependent calcineurin. 2 FPLX:PPP3 inhibits protein dephosphorylation GO:0006470 ProteinFamily dfe6b33a-bbee-11e5-9b9d-001a4ae51247 10.1016/j.brainres.2012.02.025 Involvement in HFS-induced LTP suppression of protein dephosphorylation by calcineurin, activated by the preconditioning LFS We hypothesized that Ca2+efflux through IP3Rs during or after the preconditioning LFS triggered the second messenger systems of LTP suppression (including dephosphorylation of synaptic proteins) at mossy fiber-CA3 neuron synapses without affecting synaptic transmission. 2 UNIPROT:Q13526 activates protein dephosphorylation GO:0006470 Protein 51143202-bc4f-11e5-8d2d-001a4ae51247 10.1016/S1074-5521(02)00310-1 Specifically, Pin1 binds to pSer/pThr-Pro sequences and isomerizes pSer/Thr-Pro bonds, altering the protein conformation in a phosphorylation-dependent manner and/or promoting protein dephosphorylation. 2 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily 356f8946-c6b1-11ee-9133-0050569a1f61 10.1016/j.scitotenv.2023.167167 The inhibition of PP1 and PP2A activity induces protein dephosphorylation; however, after the protein is dephosphorylated, the activity of PP1 and PP2A cannot be restored. 2 FPLX:MED activates protein dephosphorylation GO:0006470 ProteinFamily 555d90ee-bc2a-11e5-8abe-001a4ae51246 10.1016/j.bbagen.2005.07.001 We assume that the multiple serine residues in the inserted meD peptide enhance its protein dephosphorylation capability. 2 UNIPROT:Q86XI6 activates protein dephosphorylation GO:0006470 Protein 379b75d4-ca5e-11e5-8050-001a4ae51246 12805359 To better characterize the breadth of PTG-induced protein dephosphorylation, protein phosphorylation patterns were compared in control and PTG-overexpressing cells. 2 UNIPROT:P25445 activates protein dephosphorylation GO:0006470 Protein 06fb88cc-cb28-11e5-b419-001a4ae51246 11502750 Furthermore, calyculin A, an inhibitor of both PP1 and PP2A, completely blocked ceramide- and FAS-induced SR protein dephosphorylation, while okadaic acid at concentrations that only affect PP2A in cells had no effect. 2 FPLX:Integrins activates protein dephosphorylation GO:0006470 ProteinFamily 186bb454-ab8f-11e6-90f5-001a4ae51247 PMC4646280 Both integrin-mediated protein phosphorylation and integrin-mediated protein dephosphorylation may play a role in the control of Rho GTPase signaling. 2 UNIPROT:P00918 activates protein dephosphorylation GO:0006470 Protein 1ba6eb7a-ca03-11e5-9b70-001a4ae51247 15746091 These data strongly suggest that an increase in intracellular Ca2+by TRPV1 leads to down-regulation of HVACCs by protein dephosphorylation mediated by Ca2+-dependent calcineurin. 2 MESH:D010455 activates protein dephosphorylation GO:0006470 Phenotype 555d90ee-bc2a-11e5-8abe-001a4ae51246 10.1016/j.bbagen.2005.07.001 We assume that the multiple serine residues in the inserted meD peptide enhance its protein dephosphorylation capability. 2 IP:IPR001952 activates protein dephosphorylation GO:0006470 ProteinFamily 19978b22-f420-11e5-92e3-001a4ae51247 11152466 Alkaline phosphatase treatment, which induces protein dephosphorylation, revealed a detailed pattern of the tau isoforms with significant shifts in electrophoretic mobility (Fig.4c). 2 GO:0006468 activates protein dephosphorylation GO:0006470 Phenotype 379b75d4-ca5e-11e5-8050-001a4ae51246 12805359 To better characterize the breadth of PTG-induced protein dephosphorylation, protein phosphorylation patterns were compared in control and PTG-overexpressing cells. 2 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily fd38052a-c8e5-11e5-9624-001a4ae51246 17690339 "Taken together, these studies suggest that while calcineurin-mediated protein dephosphorylation is required for working memory, calcium-dependent phosphorylation is detrimental.|||(1) Calcineurin-mediated protein dephosphorylation is involved in modulating the ion channel currents necessary for delay-period activity within the prefrontal cortex." 2 UNIPROT:Q8WZ74 activates protein dephosphorylation GO:0006470 Protein 66190858-cb27-11e5-b419-001a4ae51246 PMC145406 Since E4‐ORF4 induces SR protein dephosphorylation, E4‐ORF4 might be expected to interact preferentially with hyperphosphorylated SR proteins.|||We further showed that the virus‐encoded E4‐ORF4 protein induces SR protein dephosphorylation, and has the capacity to activate IIIa pre‐mRNA splicing, bothin vitroand in transiently transfected cells (Kanopkaet al., 1998). 1 protein dephosphorylation GO:0006470 activates CHEBI:29103 Chemical 83d24dd2-bbeb-11e5-8abe-001a4ae51246 PMC3112523 Our data suggest that CaN-mediated protein dephosphorylation would be dominant compared to CaMKII-mediated phosphorylation under normal conditions, since CaN inhibitors were found to significantly suppress K+channel activity, but an inhibitor of CaMKII alone did not [13]. 1 protein dephosphorylation GO:0006470 activates GO:0051323 Phenotype 4b317e00-c486-11e5-8491-001a4ae51247 PMC3423812 "In mammalian cells, the inhibition of protein dephosphorylation induces similar alterations: the inhibition of metaphase–anaphase transition accompanied by the formation of abnormal spindles, leading to altered chromatid segregation (Vandré and Wills, 1992;Lankoffet al., 2003;Bonnesset al., 2006)." 1 protein dephosphorylation GO:0006470 inhibits GO:0006915 Phenotype eccb6670-ec2e-11e5-926d-001a4ae51247 PMC4776161 histolyticatrophozoites to Jurkat cells induced a contact-dependent protein dephosphorylation by host cell protein tyrosine phosphatases (PTPs) such as SHP-1 and SHP-2 (Kim et al., 2010), since pretreatment of cells with a PTP inhibitor inhibited amoeba-induced dephosphorylation and cell apoptosis (Teixeira and Mann, 2002). 1 protein dephosphorylation GO:0006470 activates GO:0008219 Phenotype 04cc8d00-bbdb-11e5-8abe-001a4ae51246 PMC4607873 Inhibition of protein dephosphorylation by SOV treatment blocks the downregulation of HO-1 and TG cell death caused by bacterial infection. 1 protein dephosphorylation GO:0006470 activates GO:0043583 Phenotype b37ba222-bc54-11e5-8abe-001a4ae51246 PMC4588875 Therefore, protein dephosphorylation may be very important to activate N assimilation supporting ear development in maize under LN. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 4b55f931-7f6f-11ea-8183-001a4a160175 PMC7119946 Two major pathways have been described for AT 2 receptor signaling (Nouet and Nahmias 2000) : (a) activation of protein phosphatases causing protein dephosphorylation and (b) activation of the nitric oxide (NO)/guanosine cyclic 3', 5'-monophosphate (cGMP) pathway. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 09069c32-bc3d-11e5-ac4e-001a4ae51246 PMC3597327 Phosphatase catalyzes the hydrolysis reaction to promote the process of protein dephosphorylation. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 50daec44-ca01-11e5-9b70-001a4ae51247 16288019 "To confirm the identity of the slower migrating form of the protein as a phosphorylated isoform, protein lysates were treatedin vitrowith λ phosphatase to induce nonspecific protein dephosphorylation (Fig. 4A,rightmost lanes)." 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 16aab572-c470-11e5-85e4-001a4ae51246 PMC2669206 "The fact that two of these genes were identified previously in genetic screens for cell cycle mutants (34) and that overexpression of a dominant-negative form of PphA affects growth and mitosis (26) and the identification of AnFCP1 as a potential mitotic regulator in the screen described in this study clearly demonstrate the essential importance of phosphatase-mediated protein dephosphorylation in regulating cell cycle progression." 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily ccc153c0-bbdd-11e5-8abe-001a4ae51246 PMC2442476 How calcification is impacted by specific inhibition of phosphorylation of the matrix phosphoproteins has not yet been reported, nor has the effect of preventing phosphatase-mediated protein dephosphorylation at different stages of differentiation. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 63d3d2ec-c8e0-11e5-9ad8-001a4ae51247 PMC1592722 "In contrast, the catalytic steps of splicing require protein dephosphorylation that is mediated by members of the PP1 and PP2 phosphatase families (28,30)." 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily ef433f3a-f459-11e5-92e3-001a4ae51247 PMC4806917 Some of them are essential, others are required for normal growth or involved in mitosis, which supports the importance of phosphatase-mediated protein dephosphorylation in regulating cell cycle progression and development in fungi [108,109]. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 036b9db4-5cb8-11e7-9833-001a4ae51246 PMC4762518 Kinase-induced protein phosphorylation and phosphatase-induced protein dephosphorylation are crucial for signal transduction in both prokaryotic and eukaryotic species (22–26). 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily eae8841a-bbef-11e5-9b9d-001a4ae51247 10.1016/j.phytochem.2010.12.009 Protein dephosphorylation is catalyzed by protein phosphatases. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily c92dce56-bc09-11e5-9b9d-001a4ae51247 PMC2505351 Protein dephosphorylation is catalyzed by protein phosphatases, and in humans there are three major types of protein phosphatases: tyrosine phosphatases (PTPases), serine/threonine phosphatases (PPases) and “dual specificity” phosphatases (DSPases). 1 FPLX:Phosphatase inhibits protein dephosphorylation GO:0006470 ProteinFamily 9f25a1c2-3512-11e8-bf76-001a4a160175 PMC4975672 Indirect activation of CAR Shortly after PB was discovered to be a CAR activator, Kawamoto et al. demonstrated that activation of CAR by PB was majorly related to nuclear translocation that could be blocked by the protein phosphatase inhibitor OA, suggesting that PB-mediated CAR nuclear translocation was associated with a signaling cascade that requires phosphatase-mediated protein dephosphorylation[25]. 1 FPLX:Phosphatase activates protein dephosphorylation GO:0006470 ProteinFamily 2bee4316-c9fe-11e5-9b70-001a4ae51247 15545344 Pharmacological studies suggest that type 1 protein phosphatase mediates protein dephosphorylation (8,12,15,21). 1 MESH:D003300 activates protein dephosphorylation GO:0006470 Phenotype f7482be2-bc22-11e5-8abe-001a4ae51246 PMC2386873 Copper release stimulates protein dephosphorylation, completing the cycle. 1 CHEBI:29101 inhibits protein dephosphorylation GO:0006470 Chemical 13695dfa-3750-11e8-b868-001a4a160176 28148733 To prevent protein dephosphorylation, the blocking buffer, Ab solutions, and the wash buffer were supplemented with 0.1 mM sodium orthovanadate. 1 UNIPROT:P43405 inhibits protein dephosphorylation GO:0006470 Protein 5600ff0a-5c5f-11e7-8b40-001a4ae51247 PMC5027463 "Along this line, it is possible that the CD47-SIRPα–mediated SHP activity inhibits this phagocytic receptor through protein dephosphorylation that counters the effect by Syk." 1 UNIPROT:P02724 activates protein dephosphorylation GO:0006470 Protein 64f0ba7c-c472-11e5-91a7-001a4ae51247 PMC2740580 This GPa pool presumably arises due to an inability of PTG-GL-PP1 to effectively mediate protein dephosphorylation. 1 GO:0030148 activates protein dephosphorylation GO:0006470 Phenotype b6c5d77a-ca02-11e5-be63-001a4ae51246 PMC1382306 In MOLT-4 cells, increased sphingolipid synthesis was shown to cause SR protein dephosphorylation (Jenkinset al.,2002), and SR proteins play a crucial role in splicing during heat stress (Shinet al.,2004). 1 FPLX:CAPN activates protein dephosphorylation GO:0006470 ProteinFamily dee46652-cb2b-11e5-8189-001a4ae51246 PMC127861 E. histolytica-induced cleavage of PTP1B.PTP1B may be implicated in ameba-induced protein dephosphorylation since it is activated by calpain-mediated proteolytic cleavage. 1 MESH:D054883 activates protein dephosphorylation GO:0006470 Phenotype d58dc6cc-ab89-11e6-90f5-001a4ae51247 PMC4634075 Exogenous application of9-HOTor9-KOTcombined withBLmight facilitate the oxylipin activation of theBRpathway in a larger number of cells and tissues, thus enhancing the visualization of protein dephosphorylation. 1 UNIPROT:P10145 inhibits protein dephosphorylation GO:0006470 Protein 9e4cb0a0-cb2a-11e5-b419-001a4ae51246 12456487 In some experiments, NaF (20 mmol/L) and okadaic acid (1 μmol/L) were added to the homogenization medium to prevent protein dephosphorylation that might occur during homogenization. 1 GO:0000372 activates protein dephosphorylation GO:0006470 Phenotype 66190858-cb27-11e5-b419-001a4ae51246 PMC145406 Discussion We have shown previously that E4‐ORF4 activates IIIa pre‐mRNA splicing bothin vivoandin vitroand, furthermore, induces SR protein dephosphorylation (Kanopkaet al., 1998). 1 GO:0060291 inhibits protein dephosphorylation GO:0006470 Phenotype dfe6b33a-bbee-11e5-9b9d-001a4ae51247 10.1016/j.brainres.2012.02.025 Involvement in HFS-induced LTP suppression of protein dephosphorylation by calcineurin, activated by the preconditioning LFS We hypothesized that Ca2+efflux through IP3Rs during or after the preconditioning LFS triggered the second messenger systems of LTP suppression (including dephosphorylation of synaptic proteins) at mossy fiber-CA3 neuron synapses without affecting synaptic transmission. 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 2105c140-b992-11ee-a19b-0050569a791b 10.1007/s10725-019-00540-z TheActingene was used as an internal control Discussion discussion Protein dephosphorylation mediated by serine/threonine protein phosphatase PP2A plays an important role in cellular signaling. 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily ad4f7c67-dbdd-11ea-a074-001a4a160175 32074998 In the mammalian brain, PP2A-mediated protein dephosphorylation plays an important role in learning and memory. 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily c1b53b80-3387-11e8-bf76-001a4a160175 27812910 PP2A-mediated protein dephosphorylation has been implicated in several developmental programs and acclimation processes (País et al.38), including seed germination (Chang et al.6), formative cell division regulation in roots (Yue et al.66), cytoskeleton control (Camilleri et al.4), cold acclimation (Monroy et al.30), drought tolerance (Xu et al.64), wounding (Rojo et al.46) and plant defense responses (He et al.15; Trotta et al.57). 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily e089f01e-bc3d-11e5-8d2d-001a4ae51247 PMC3080859 Our data indicate that RCN1-containing PP2A complexes dephosphorylate and promote the turnover of type 1 ACS isozymes in etiolated seedlings, suggesting that PP2A-mediated protein dephosphorylation is an important counterbalance to MAPK action. 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 6a3dc216-bc18-11e5-9b9d-001a4ae51247 10.1016/j.yjmcc.2013.09.006 PP1 appears to preferentially dephosphorylate Ser23/24 on TnI, whereas PP2A induces a more uniform protein dephosphorylation. 1 FPLX:PPP2 inhibits protein dephosphorylation GO:0006470 ProteinFamily 356f8946-c6b1-11ee-9133-0050569a1f61 10.1016/j.scitotenv.2023.167167 The inhibition of PP1 and PP2A activity induces protein dephosphorylation; however, after the protein is dephosphorylated, the activity of PP1 and PP2A cannot be restored. 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 77b61a34-c478-11e5-85e4-001a4ae51246 PMC3135915 "Experiments with the phosphatase inhibitor okadaic acid have implicated a role for PP2A-mediated protein dephosphorylation in a range of developmental programs and acclimation processes, including seed germination (Chang et al., 1999), cold acclimation (Monroy et al., 1998), and wounding (Rojo et al., 1998)." 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily 63d3d2ec-c8e0-11e5-9ad8-001a4ae51247 PMC1592722 "In contrast, the catalytic steps of splicing require protein dephosphorylation that is mediated by members of the PP1 and PP2 phosphatase families (28,30)." 1 FPLX:PPP2 activates protein dephosphorylation GO:0006470 ProteinFamily be308c14-c8de-11e5-a1fd-001a4ae51246 16428343 Okadaic acid enhanced and calyculin A blocked eNOS phosphorylation at Ser1177 and dephosphorylation of eNOS at Thr495 and endothelial NO production induced by forskolin, indicating both PP1- and PP2A-mediated protein dephosphorylation plays an important role in the regulation of eNOS activation. 1 UNIPROT:P08246 activates protein dephosphorylation GO:0006470 Protein a6b6cb70-bc45-11e5-9b9d-001a4ae51247 PMC4586250 In normal cells, low HNE concentrations activate TCRviaCSB protein dephosphorylation. 1 UNIPROT:P50052 activates protein dephosphorylation GO:0006470 Protein 4bf47cfa-c8e8-11e5-a1fd-001a4ae51246 17951304 Unlike AT1 receptor, the AT2 receptor is coupled to various phosphatases and mediates protein dephosphorylation. 1 CHEBI:29036 activates protein dephosphorylation GO:0006470 Chemical 735fd27e-c47b-11e5-85e4-001a4ae51246 PMC3143623 The release of copper from the intramembrane sites into the lumen of thetrans-Golgi or vesicles triggers protein dephosphorylation (2,4,5). 1 MESH:D004229 inhibits protein dephosphorylation GO:0006470 Phenotype 44caf8bc-cb8d-11e5-a7a8-001a4ae51246 10988296 "In agreement with this concept, the reducing agent DTT significantly stimulated the activity of the cytosolic protein tyrosine phosphatase SHP-2in vitro, suggesting that a reducing environment, as found in confluent cells, can promote protein dephosphorylation through the direct activation of protein tyrosine phosphatases (Fig.6C)." 1 UNIPROT:P01308 activates protein dephosphorylation GO:0006470 Protein d5f7c9b6-cca3-11e5-aade-001a4ae51247 9045612 Although the signaling pathways linking the insulin receptor to PP1 activation remain uncertain (3), the catalytic subunit of PP1 (PP1C) is thought to be maintained at discrete cellular locations in order to ensure the specificity of protein dephosphorylation produced by insulin (4). 1 UNIPROT:P09923 activates protein dephosphorylation GO:0006470 Protein 9e7e3a40-bc1a-11e5-8abe-001a4ae51246 PMC3526294 The phosphorylation status of both Dicer (our unpublished data) and CLIMP-63 (28) proteins did not seem to influence their ability to interact together, as the presence of phosphatase inhibitors, which preserved protein phosphorylation, or calf intestinal alkaline phosphatase (CIAP), which induced protein dephosphorylation, did not modify the interaction (Figure 2C, lanes 1–7). 1 UNIPROT:P09923 activates protein dephosphorylation GO:0006470 Protein c09d5814-bbe2-11e5-8abe-001a4ae51246 10.1016/j.bbagrm.2013.04.004 To investigate the influence of the phosphorylation status of HNF1α on 14-3-3ζ binding, we first performed GST-pulldown in the presence of calf intestinal alkaline phosphatase (CIP), which could contribute to protein dephosphorylation in cell-free extracts. 1 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily 83d24dd2-bbeb-11e5-8abe-001a4ae51246 PMC3112523 Our data suggest that CaN-mediated protein dephosphorylation would be dominant compared to CaMKII-mediated phosphorylation under normal conditions, since CaN inhibitors were found to significantly suppress K+channel activity, but an inhibitor of CaMKII alone did not [13]. 1 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily 22de3480-cceb-11e5-b6ad-001a4ae51246 8663324 "Several examples of depolarization-induced, calcineurin-mediated protein dephosphorylation events have been recognized in the neuron." 1 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily 8daabc84-bc2e-11e5-8abe-001a4ae51246 10.1016/S0166-2236(00)01930-5 They also block Ca2+-dependent AMPA receptor internalization in postsynaptic dendrites43and transferrin internalization in non-neuronal cells44, indicating that receptor-mediated endocytosis (RME) might also be dependent on CaN-mediated protein dephosphorylation. 1 FPLX:PPP3 activates protein dephosphorylation GO:0006470 ProteinFamily d9d74622-c471-11e5-9da3-001a4ae51247 PMC2836045 Alternatively, Yam8p channel activity might also be controlled by protein dephosphorylation catalyzed by calcineurin (63). 1 MESH:D007511 activates protein dephosphorylation GO:0006470 Phenotype 952449ac-bbdf-11e5-8abe-001a4ae51246 10.1016/j.neulet.2007.11.073 1A and 2A-D), indicates that ischemia alone leads to protein dephosphorylation. 1 MESH:D000242 activates protein dephosphorylation GO:0006470 Phenotype ee7c5d4c-ca5c-11e5-8050-001a4ae51246 12724359 FMRFamide also reverses S channel closure produced by 5-HT or cAMP (Belardetti et al. 1987) by stimulating protein dephosphorylation (Endo et al. 1995;Sweatt et al. 1989). 1 MESH:D019163 activates protein dephosphorylation GO:0006470 Phenotype 44caf8bc-cb8d-11e5-a7a8-001a4ae51246 10988296 "In agreement with this concept, the reducing agent DTT significantly stimulated the activity of the cytosolic protein tyrosine phosphatase SHP-2in vitro, suggesting that a reducing environment, as found in confluent cells, can promote protein dephosphorylation through the direct activation of protein tyrosine phosphatases (Fig.6C)." 1 UNIPROT:P05451 activates protein dephosphorylation GO:0006470 Protein 36a4421c-c470-11e5-9da3-001a4ae51247 19420001 PTK-mediated tyrosine phosphorylation is generally an activating event in signaling pathways, and it is apparent that intertwined PTP-mediated protein dephosphorylation events are also required for signal transduction and propagation (26). 1 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 84f2a2c2-bc3e-11e5-8d2d-001a4ae51247 10.1016/S0306-4522(01)00114-2 One of these agents, okadaic acid, inhibits types Ia and IIa protein phosphatase enzymes, prevents protein dephosphorylation and permits uncountered phosphorylation by protein kinases. 1 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 36bfae12-bbfb-11e5-8abe-001a4ae51246 10.1016/j.jplph.2011.05.004 In contrast to protein phosphorylation inhibited by K252a, protein dephosphorylation inhibited by OA is likely to variously function in guard cells of different varieties and species. 1 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 9e4cb0a0-cb2a-11e5-b419-001a4ae51246 12456487 In some experiments, NaF (20 mmol/L) and okadaic acid (1 μmol/L) were added to the homogenization medium to prevent protein dephosphorylation that might occur during homogenization. 1 CHEBI:44658 inhibits protein dephosphorylation GO:0006470 Chemical 144e8fd2-bc47-11e5-9b9d-001a4ae51247 PMC3115801 By inhibiting protein phosphatase 1 and 2A (Cohen et al., 1990), OA stimulation decreases protein dephosphorylation and results in the elevated phosphorylation state of proteins. 1 FPLX:PPP1 inhibits protein dephosphorylation GO:0006470 ProteinFamily 356f8946-c6b1-11ee-9133-0050569a1f61 10.1016/j.scitotenv.2023.167167 The inhibition of PP1 and PP2A activity induces protein dephosphorylation; however, after the protein is dephosphorylated, the activity of PP1 and PP2A cannot be restored. 1 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily fa533de8-c480-11e5-a92e-001a4ae51246 PMC3378076 Activation requires protein dephosphorylation that is mediated by the PP1 phosphatases GSP-1 and GSP-2 (42). 1 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily d1872dc8-bbd6-11e5-956b-001a4ae51247 PMC2768498 Many of the metabolic effects of insulin are also mediated by protein dephosphorylation catalyzed mainly by protein phosphatase-1 (PP1) (Brady and Saltiel, 2001). 1 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily 559ca186-bc1a-11e5-9b9d-001a4ae51247 PMC3351148 C6 pyridinium ceramide (PyrCer) inhibits PP1 It has been previously reported that ceramides promote PP1 activity (13) and that exogenousd-(e)-C6-ceramide causes SR-protein dephosphorylation (24). 1 FPLX:PPP1 activates protein dephosphorylation GO:0006470 ProteinFamily 63d3d2ec-c8e0-11e5-9ad8-001a4ae51247 PMC1592722 "In contrast, the catalytic steps of splicing require protein dephosphorylation that is mediated by members of the PP1 and PP2 phosphatase families (28,30)." 1 FPLX:PPP1 inhibits protein dephosphorylation GO:0006470 ProteinFamily 8ca8059e-3534-11e9-9cf0-001a4a160175 PMC5899473 Rather, PP1 constitutes the catalytic unit of a large array of multisubunit holoenzymes, which regulate and target PP1 activity to prevent uncontrolled protein dephosphorylation and cell death (48). 1 UNIPROT:Q06529 activates protein dephosphorylation GO:0006470 Protein 8b0e0e70-cd29-11e5-888a-001a4ae51246 8530387 "FC-mediated Intracellular Signaling A truly novel aspect of the data in this report is the finding that FC can trigger intracellular protein dephosphorylation (Fig. 7)." 1 UNIPROT:P25445 activates protein dephosphorylation GO:0006470 Protein bd2eddae-ca03-11e5-ab20-001a4ae51246 16000324 Fas activation is also known to cause SR protein dephosphorylation (73), so there may be complex regulatory networks controlling apoptosis involving alternative splicing of HipK3 (74). 1 FPLX:PKC inhibits protein dephosphorylation GO:0006470 ProteinFamily 9859de3a-ca02-11e5-ab20-001a4ae51246 16338977 Additional experiments were performed to explore whether enhanced PKC-dependent protein phosphorylation and/or reduced protein dephosphorylation triggers phosphatidylserine exposure of erythrocytes. 1 UNIPROT:P01100 activates protein dephosphorylation GO:0006470 Protein 30d4cd6a-c8e0-11e5-9ad8-001a4ae51247 17026529 The inhibitory effect of C2-ceramide was significantly reduced by the PP1 and PP2A inhibitor, okadaic acid, indicating that C2-ceramide-induced c-Fos expression necessitates protein dephosphorylation. 1 MESH:D000255 inhibits protein dephosphorylation GO:0006470 Phenotype 55b9da36-ca01-11e5-be63-001a4ae51246 16306126 ATP depletion activates CLH-3b by inducing net protein dephosphorylation (28). 1 MESH:D000255 inhibits protein dephosphorylation GO:0006470 Phenotype 0a0ce868-bbe1-11e5-8abe-001a4ae51246 10.1016/S0168-9452(01)00328-4 It has been suggested[5]that these treatments induce, via inhibition of ATP-driven proton pumps, a cytosolic acidification and a concomitant stimulation of protein dephosphorylation. 1 UNIPROT:Q5VWP3 activates protein dephosphorylation GO:0006470 Protein c09d5814-bbe2-11e5-8abe-001a4ae51246 10.1016/j.bbagrm.2013.04.004 To investigate the influence of the phosphorylation status of HNF1α on 14-3-3ζ binding, we first performed GST-pulldown in the presence of calf intestinal alkaline phosphatase (CIP), which could contribute to protein dephosphorylation in cell-free extracts. 1 UNIPROT:O14492 inhibits protein dephosphorylation GO:0006470 Protein 540546bd-f58e-11eb-959c-001a4a160176 30125824 Recently, Fu et al. reported that APS could effectively inhibit sperm protein dephosphorylation caused by ROS through the cAMP-PKA pathway [14]. 1 MESH:D013110 activates protein dephosphorylation GO:0006470 Phenotype 204c7246-bc28-11e5-8abe-001a4ae51246 PMC2727905 Our finding that active Pak1 induces dephosphorylation of cTnI and C-protein through activation of PP2A (Ke et al 2004) provides a plausible mechanism for induction of protein dephosphorylation by sphingosine. 1 UNIPROT:P17219 activates protein dephosphorylation GO:0006470 Protein 3cd89dfc-f586-11eb-8a6a-001a4a160175 30668972 Effect of PTTH on the tyrosine dephosphorylation of PP2Acand its developmental changes Although PTTH treatment did not affect the protein levels of each subunit, it is unclear whether or not PTTH increases the protein dephosphorylation of the PP2Acsubunit. 1 MESH:D012701 activates protein dephosphorylation GO:0006470 Phenotype ee7c5d4c-ca5c-11e5-8050-001a4ae51246 12724359 FMRFamide also reverses S channel closure produced by 5-HT or cAMP (Belardetti et al. 1987) by stimulating protein dephosphorylation (Endo et al. 1995;Sweatt et al. 1989). 1 UNIPROT:P60484 activates protein dephosphorylation GO:0006470 Protein eecd8c8a-bc47-11e5-ac4e-001a4ae51246 10.1016/j.bbapap.2010.05.009 In summary, even though both the phospholipid and phospho protein dephosphorylation reactions catalyzed by PTEN likely follow the same catalytic mechanism, our results indicate that there are significant differences in the requirements of the active site configuration for the two types of phosphatase activities. 1 GO:0006468 inhibits protein dephosphorylation GO:0006470 Phenotype 9859de3a-ca02-11e5-ab20-001a4ae51246 16338977 Additional experiments were performed to explore whether enhanced PKC-dependent protein phosphorylation and/or reduced protein dephosphorylation triggers phosphatidylserine exposure of erythrocytes. 1 CHEBI:15794 inhibits protein dephosphorylation GO:0006470 Chemical 8450f836-c8ec-11e5-8b47-001a4ae51246 18539601 Juglone Prevents Postmitotic Protein Dephosphorylation in the Absence of Active Spindle Checkpoint Factors—To investigate a direct role of the mitotic spindle checkpoint in the abolished degradation of cyclin B1 and the inhibited dephosphorylation of mitotic phosphoproteins, we examined the activity of spindle checkpoint factors in juglone-treated cells. 1 CHEBI:46979 activates protein dephosphorylation GO:0006470 Chemical 30d4cd6a-c8e0-11e5-9ad8-001a4ae51247 17026529 The inhibitory effect of C2-ceramide was significantly reduced by the PP1 and PP2A inhibitor, okadaic acid, indicating that C2-ceramide-induced c-Fos expression necessitates protein dephosphorylation. 1 MESH:D001424 activates protein dephosphorylation GO:0006470 Phenotype 04cc8d00-bbdb-11e5-8abe-001a4ae51246 PMC4607873 To examine whether protein dephosphorylation induced by bacterial infection contributes to cell death, infection ofL. 1 UNIPROT:P23647 inhibits protein dephosphorylation GO:0006470 Protein 540546bd-f58e-11eb-959c-001a4a160176 30125824 Recently, Fu et al. reported that APS could effectively inhibit sperm protein dephosphorylation caused by ROS through the cAMP-PKA pathway [14]. 1 UNIPROT:O75807 activates protein dephosphorylation GO:0006470 Protein 5f73780e-bc4e-11e5-8d2d-001a4ae51247 PMC3834229 CHOP-mediated activation of GADD34 promotes protein dephosphorylation of elF2α reversing translational inhibition[18]. 1 UNIPROT:Q7M3Y3 activates protein dephosphorylation GO:0006470 Protein 6a3dc216-bc18-11e5-9b9d-001a4ae51247 10.1016/j.yjmcc.2013.09.006 PP1 appears to preferentially dephosphorylate Ser23/24 on TnI, whereas PP2A induces a more uniform protein dephosphorylation. 1 UNIPROT:Q5AVR1 inhibits protein dephosphorylation GO:0006470 Protein 40e13780-bc0f-11e5-8abe-001a4ae51246 PMC2807945 CalA was added to the incubation buffer to prevent protein dephosphorylation during incubation. 1 UNIPROT:P08922 inhibits protein dephosphorylation GO:0006470 Protein 8ef162e6-c471-11e5-85e4-001a4ae51246 19755521 "ROS are able to transiently inhibit PTPs through the reversible oxidization of the catalytic cysteine residue, thereby suppressing protein dephosphorylation (Meng et al., 2002)." 1 UNIPROT:P08922 activates protein dephosphorylation GO:0006470 Protein 540546bd-f58e-11eb-959c-001a4a160176 30125824 Recently, Fu et al. reported that APS could effectively inhibit sperm protein dephosphorylation caused by ROS through the cAMP-PKA pathway [14]. 1 GO:0008380 activates protein dephosphorylation GO:0006470 Phenotype 2772638a-bc25-11e5-8abe-001a4ae51246 PMC4346872 However, as described above, E4-ORF4 is a regulator of alternative RNA splicing by causing SR protein dephosphorylation [26,27].