count	source_label	source_id	relationship	target_label	target_id	entity_type	solr_id	publication_id	sentences
16		CHEBI:16836	activates	4-nitrophenyl acetate	CHEBI:82635	Chemical	2996040a-340a-11e8-9fbf-001a4a160176	27871381	The degree of inhibition (Abs (%)) due to the sulfonamide inhibitors was evaluated according to the equation:Abs(%)=Abs0−Abs1Abs0×100Where: Abs0and Abs1are the absorbance response of 4-nitrophenol which is the enzymatic product of 4-nitrophenyl acetate (NPA) at 405nm for 90min in Tris-HCl buffer recorded in absence and in presence of CA inhibitors.|||Optimization of the measurement The carbonic anhydrase activities were measured by monitoring the 4-nitrophenol, which is the enzymatic product of 4-nitrophenyl acetate (NPA) at 405nm for 90min in Tris-HCl buffer.Fig.|||Multiphotometric assay measurements The carbonic anhydrase activities were measured by monitoring the 4-nitrophenol, which is the enzymatic product of 4-nitrophenyl acetate (NPA) at 405nm for 90min in Tris-HCl buffer.Fig.|||The carbonic anhydrase activities were measured by monitoring the 4-nitrophenol, which is the enzymatic product of 4-nitrophenyl acetate (NPA) at 405nm for 90min in Tris-HCl buffer.Fig.|||Results and discussions The carbonic anhydrase activities were measured by monitoring the 4-nitrophenol, which is the enzymatic product of 4-nitrophenyl acetate (NPA) at 405nm for 90min in Tris-HCl buffer.Fig.
4		CHEBI:78036	activates	4-nitrophenyl acetate	CHEBI:82635	Chemical	75f73a3c-352a-11e8-9fbf-001a4a160176	25769102	Chemicals Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.|||Material and methods Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.|||Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.
4		CHEBI:53001	activates	4-nitrophenyl acetate	CHEBI:82635	Chemical	75f73a3c-352a-11e8-9fbf-001a4a160176	25769102	Chemicals Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.|||Material and methods Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.|||Cd(NO3)2×4H2O, AgNO3, Cu(NO3)2×2.5H2O, NiSO4, CNBr-activated Sepharose 4B, protein assay reagents, 4-nitrophenylacetate and chemicals for electrophoresis were purchased from Sigma-Aldrich Co. Para-aminobenzene sulfonamide andl-tyrosine were from Merck.
2	4-nitrophenyl acetate	CHEBI:82635	activates		IP:IPR000801	ProteinFamily	5f349554-3905-11e8-9fbf-001a4a160176	28935566	Esterase activity of BplA and hybrid VLPs Esterase activity was measured by mixing 100μL of protein sample with 850μL of PBS pH 7.4 and the reaction is triggered by the addition of 50μL of 4-Nitrophenyl acetate (4NFA) 10mM dissolved in acetonitrile.
1	4-nitrophenyl acetate	CHEBI:82635	activates		CHEBI:16836	Chemical	d7b39536-cddb-11ee-b346-0050569a791b	10.1016/j.molcatb.2014.08.009	Catalytic tests of enzymes by hydrolysis of 4-nitorophenyl acetate Hydrolytic activities of enzymes were performed by measuring the increase of 4-nitrophenol produced by the hydrolysis of 4-nitrophenyl acetate.
1	4-nitrophenyl acetate	CHEBI:82635	activates		CHEBI:77700	Chemical	f03f2e38-bc48-11e5-9b9d-001a4ae51247	10.1016/j.poly.2011.03.040	Treatment of 4-nitrophenyl acetate and methanol in the presence of1–6at 50°C produced methyl acetate quantitatively within 5days for1, 8days for2, 7days for3, 0.13days for4, 8days for5and 11days for6, under neutral conditions (Eq.
1	4-nitrophenyl acetate	CHEBI:82635	inhibits		IP:IPR000801	ProteinFamily	4b0a9b04-6c70-11e6-a7ed-001a4ae51246	PMC5000742	Mechanistically, the esterase activity of hDJ-1 requires oxidation as an oxido-mimetic mutant of hDJ-1 (C016D) is constitutively active towards 4-nitrophenyl acetate (pNPA) whereas a PD-associated mutant, known to reduce oxidation of cysteines in hDJ-1, suppresses its esterase activity.
1		PF:PF01083	activates	4-nitrophenyl acetate	CHEBI:82635	ProteinFamily	78b6affa-c69c-11ee-8b99-0050569a1f61	10.1016/j.chemosphere.2023.140661	Immobilization of lipase, and cutinase in SiO2nanoparticles caused the biodegradation of 4-nitrophenyl acetate (4-NPA) to 4-nitrophenol (4-NP), and acetic acid (Eva et al., 2021) in the presence of water molecule.
1		PF:PF00151	activates	4-nitrophenyl acetate	CHEBI:82635	ProteinFamily	78b6affa-c69c-11ee-8b99-0050569a1f61	10.1016/j.chemosphere.2023.140661	Immobilization of lipase, and cutinase in SiO2nanoparticles caused the biodegradation of 4-nitrophenyl acetate (4-NPA) to 4-nitrophenol (4-NP), and acetic acid (Eva et al., 2021) in the presence of water molecule.